Vol. 1 No. 1-4: In SITU HYBRIDIZATION and immunohistochemical technique for Epstein- Barr Virus (EBV) detection in Misan province breast cancer women
Alaa Shamikh Hassan1, Thaer Saleh Sabor Al-Omary2*, Saud Kadhim Abbas3
1Department of Pathology, College of Medicine, University of Misan, Misan, Iraq
2Department of Surgery, College of Medicine, University of Misan, Misan, Iraq
3Department of Surgery, College of Medicine, University of Misan, Misan, Iraq
Abstract
Breast cancer continues to be the most common malignancy and the primary cause of cancer-related mortality among women globally. Although conventional risk factors inadequately account for a considerable number of cases, recent findings associate viral infections, particularly Epstein-Barr Virus (EBV), with the onset and advancement of breast cancer. This research examines the existence and function of EBV in breast cancer among women in Misan Province, Iraq. Immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) were employed to analyze 70 formalin-fixed, paraffin-embedded breast tissue blocks, comprising 45 malignant and 25 non-malignant samples. The results indicated a prevalent incidence of invasive ductal carcinoma (IDC) (93.3%) among malignant cases, with EBV EBER RNA signals identified in 62% of breast cancer samples against 10% in controls, demonstrating statistically significant differences (p < 0.04). Nuclear brown staining indicative of EBV was identified in 68% of cancer tissues using IHC. The data indicate a possible correlation between EBV infection and breast cancer, underscoring the necessity for additional research into its oncogenic mechanisms and implications for preventative and treatment approaches.
In SITU HYBRIDISATION and immunohistochemical technique for Ebstain Barr Virus (EBV) detection in Misan province breast cancer women